New methods for expediting the discovery of drug targets and increase current understanding of the optimal pharmacology for treating zoonotic-parasite infections would help to reassess current therapies and develop new effective drugs. Despite zoonotic parasites have become a great risk for both Canadians and livestock, their study has undergone neglected for many years. In this way, our research team is trying to tackle major zoonotic parasites (mainly Cryptosporidium, Giardia and Leishmania) by means of innovative therapeutic approaches while understanding the mechanisms underlying drug resistance and ruling host-pathogen interactions, with an special focus on exosomes.